Fig. 1

Cardio-metabolic parameters and redox signals at RVLM in young offspring exposed to maternal HFD or ND. Dams were exposed to a 60% HFD or ND during gestation and lactation. Both HFD and ND offspring returned to ND immediate after weaning (at age of 3 weeks). a SBP measured using tail-cuff plethysmography under conscious condition; b sympathetic vasomotor activity represented by power density of low frequency component of SBP signals; c plasma NE determined by high performance liquid chromatography; d protein expression of leptin in RVLM assessed by Western blot; e tissue level of ROS measured by dihydroethidium signal; f MDA determined by colorimetric spectrophotometry; and g NOx evaluated based on chemiluminescence reaction in RVLM. Analysis was performed on tissues collected bilaterally from individual RVLM. All measurements were made in ND (n = 6–12) and HFD (n = 6–12) offspring at age of 6, 8, 10, 11 and/or 12 weeks. Data are presented as mean ± SEM. *P < 0.05 versus ND group at comparable age in post hoc Tukey’s multiple range analysis. There is no significant difference between data shown in G in one-way ANOVA