Fig. 2
Expressions of AT1R, molecules involved in redox homeostasis and leptin in RVLM of young offspring exposed to maternal HFD or ND. Representative gels and densitometric analysis of results from Western blot showing changes in protein expression of (a) AT1R, (b) gp91phox (c) SOD2, and (d) leptin or (e) ROS level in RVLM of ND (n = 6–10) or HFD (n = 6–10) offspring, alone or with additional treatment with a HMG-CoA reductase inhibitor, simvasatatin (5 mg·kg− 1·day− 1), administered via gastric gavage at age of 8 weeks for 4 weeks. Analysis was performed on tissues collected bilaterally from individual RVLM at age of 12 weeks. Data on protein expression were normalized to the average ND control value, which is set to 1.0, and are presented as mean ± SEM. *P < 0.05 versus ND group, #P < 0.05 versus HFD group in post hoc Newman-Keuls multiple-range test