Fig. 3

Mitochondrial biogenesis in RVLM of young offspring exposed to maternal HFD or ND. Representative gels (insets) and densitometric analysis of results from Western blot showing changes in protein expression of (a) PGC-1α (n = 10 per group) and (b) TFAM (n = 8 per group), two key molecules in transcription and replication of mitochondrial DNA; (c) mitochondrial DNA copy number (n = 10 per group); and (d) tissue content of ATP (n = 6 per group) in RVLM of ND or HFD offspring, alone or with additional treatment with simvastatin (5 mg·kg^− 1·day^− 1), administered via gastric gavage at age of 8 weeks for 4 weeks. Analysis was performed on tissues collected bilaterally from individual RVLM at age of 12 weeks. Data on protein expression were normalized to the average ND control value, which is set to 1.0, are presented as mean ± SEM. *P < 0.05 versus ND group ^#P < 0.05 versus HFD group in post hoc Newman-Keuls multiple-range test