Fig. 1
From: The endothelium, a key actor in organ development and hPSC-derived organoid vascularization
Strategies for the vascularization of hPSC-derived organoids. hPSC-derived endothelial cells (ECs) present in the vascularized organoids can be obtained by a) codifferentiation of human pluripotent stem cells (hPSCs) into all the cell lineages forming the organoid or b) independent differentiation of hPSCs into ECs and tissue progenitors for further coculture. hPSC-derived ECs can also be added to preformed organoids. PPs: pancreas progenitors; NP: nephron progenitors; NSCs: neural stem cells; LSECs: liver sinusoidal endothelial cells; and CECS: cerebral endothelial cells