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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer

Fig. 1

Overall workflow for the analyses of the proteome and phosphoproteome regulated by ZNF322A in lung cancer cells. a Experimental strategy for quantitative global proteomic profiling in response to silencing of ZNF322A (siZNF322A) in A549 cells. Protein extracts obtained from the transfected cells were digested, iTRAQ labeled, and analyzed with mass spectrometry. b Experimental strategy for quantitative phosphoproteomic profiling in response to siZNF322A in A549 cells. Protein extracts obtained from the transfected cells were digested, dimethyl labeled, phosphopeptide enriched, SCX fractionated, and analyzed with mass spectrometry. Construction of ZNF322A-perturbed functional network, and protein-protein interaction and the biological processes in lung cancer cells were validated by functional assays

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