Fig. 3
From: The impact of PD-L1 N-linked glycosylation on cancer therapy and clinical diagnosis
A diagram of PD-L1 antigen retrieval through sample deglycosylation. The glycan structure of PD-L1 hinders antibody-based detection targeting PD-L1 antigenic region. Sample deglycosylation is a method by pretreating fixed samples on FFPE tissue slides with recombinant N-glycosidase PNGase F to remove N-glycan moieties from polypeptides. This process renders PD-L1 antigenic region more accessible to antibody binding, which results in enhanced PD-L1 signal intensity and binding affinity, leading to improved PD-L1 detection and therapeutic correlation in clinical settings. Not drawn to scale