Fig. 1
The survival of RS218 strains with and without cjrABC-senB in the mouse bloodstream and human serum. (a) Independent infections of mice with WT-RS218 and ∆cjr-RS218 (1 × 107 CFU/mouse). Bacterial blood counts were determined at 14 h post-inoculation. (b) Coinfection of mice with equal numbers of ∆cjr-RS218 and ∆lacZ-RS218 (1 × 107 CFU/strain/mouse). Bacterial blood counts were determined at 14 h post-inoculation. (c) Coinfection of mice with equal numbers of ∆cjr-RS218/pCL1920 and ∆cjr∆lacZ-RS218/pCL1920-cjrABC-senB. Blood counts were determined at 14 h post-infection. The horizontal bars represent the median values. For (b) and (c) the bacterial counts derived from same animals are connected with lines. (d) and (e) The serum survival of the RS218 strains with and without cjrABC-senB (1 × 107 CFU/ml) after 1 h and 3 h incubation in 40% NHS (d) and 40% HI-NHS(e). The results are shown as the means ± standard deviations, and the data are representative of three independent experiments performed in triplicate. *, P value < 0.05; **, P value < 0.01; ***, P value < 0.001