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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: cjrABC-senB hinders survival of extraintestinal pathogenic E. coli in the bloodstream through triggering complement-mediated killing

Fig. 7

Survival of the RS218 strains harboring individual genes of the cjrABC-senB gene cluster in NHS or HI-NHS and localization of CjrA. (a) and (b) The survival rates of the bacterial strains were determined after the strains were incubated for 3 h in 40% NHS (a) or 40% HI-NHS (b). The data are shown as relative survival rates compared with those of Cjr-RS218. The results are shown as the means ± standard deviations, which are representative of three independent experiments performed in triplicate. (c) The localization of CjrA. The outer and inner membrane (OM and IM) fractions of CjrA-RS218, which expresses C-terminally His6-tagged CjrA, were isolated and subjected to Western blot analysis with an anti-His6 antibody or OmpA antiserum. OmpA serves as an OM marker. *, P value < 0.05

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