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Fig. 8 | Journal of Biomedical Science

Fig. 8

From: cjrABC-senB hinders survival of extraintestinal pathogenic E. coli in the bloodstream through triggering complement-mediated killing

Fig. 8

The effects of cjrABC-senB expression on the OM properties of the E. coli strain RS218. (a) SDS sensitivity assays of Cjr-RS218, Cjr+-RS218, and Cjr-RS218. The bacterial cultures were adjusted to O.D.600 = 1 in LB with different concentrations of SDS. After 2 h of incubation at 37 °C, the turbidity (O.D.600) of each bacterial culture was determined. (b) An RNase assay with Cjr-RS218, Cjr+-RS218, and CjrA-RS218. The bacteria were grown on an RNase test agar plate. The pink halos around the colonies indicate the leakage of periplasmic RNase into the agar. (c) Rifampicin sensitivity assay. Cjr-RS218, Cjr+-RS218, and CjrA-RS218 (5 × 105 CFU/ml) were cultured in Mueller-Hinton broth containing different concentrations of rifampicin in a 96 well plate (200 μl/ well). After 8 h of incubation at 37 °C, the turbidity (O.D.600) of each bacterial culture was determined. For (a) and (c) the results are shown as the mean ± standard deviation, and the data are representative of three independent experiments performed in triplicate. The asterisks indicate significant differences (*, P value < 0.05; **, P value < 0.01; ***, P value < 0.001) between the O.D.600 value of the indicated strain and that of Cjr-RS218

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