Table 1 E. coli strains and plasmids used in this study
Strains or plasmids | Relevant information | Sources |
|---|---|---|
E. coliStrains | ||
 RS218 (WT-RS218) | E. coli K1 RS218 isolated from the cerebrospinal fluid of a neonate with meningitis. | |
∆lacZ-RS218 | RS218 with a lacZ deletion (The otherwise WT-RS218) | [18] |
∆cjr-RS218 | RS218 with a cjrABC-senB deletion | This study |
∆cjr∆lacZ-RS218 | ∆cjr-RS218 with a lacZ deletion | This study |
 Cjr+-RS218 | ∆cjr-RS218 harboring pCL1920-cjrABC-senB | This study |
 Cjr+-∆lacZ-RS218 | Cjr+-RS218 with a lacZ deletion | This study |
 Cjr−-RS218 | ∆cjr-RS218 harboring pCL1920 | This study |
 CjrA-RS218 | ∆cjr-RS218 harboring pCL1920-cjrA | This study |
 CjrB-RS218 | ∆cjr-RS218 harboring pCL1920-cjrB | This study |
 CjrC-RS218 | ∆cjr-RS218 harboring pCL1920-cjrC | This study |
 SenB-RS218 | ∆cjr-RS218 harboring pCL1920-senB | This study |
Plasmids | ||
 pCL1920 | Low-copy-number plasmid | [19] |
 pCL1920-cjrABC-senB | pCL1920 harboring cjrABC-senB, which is under the control of the lac promoter on the plasmid | This study |
 pCL1920-cjrA | pCL1920 harboring cjrA with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. | This study |
 pCL1920-cjrB | pCL1920 harboring cjrB with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. | This study |
 pCL1920-cjrC | pCL1920 harboring cjrC with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. | This study |
 pCL1920-senB | pCL1920 harboring senB with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. | This study |