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Table 1 E. coli strains and plasmids used in this study

From: cjrABC-senB hinders survival of extraintestinal pathogenic E. coli in the bloodstream through triggering complement-mediated killing

Strains or plasmids Relevant information Sources
E. coliStrains
 RS218 (WT-RS218) E. coli K1 RS218 isolated from the cerebrospinal fluid of a neonate with meningitis. [16, 17]
∆lacZ-RS218 RS218 with a lacZ deletion (The otherwise WT-RS218) [18]
∆cjr-RS218 RS218 with a cjrABC-senB deletion This study
∆cjr∆lacZ-RS218 ∆cjr-RS218 with a lacZ deletion This study
 Cjr+-RS218 ∆cjr-RS218 harboring pCL1920-cjrABC-senB This study
 Cjr+-∆lacZ-RS218 Cjr+-RS218 with a lacZ deletion This study
 Cjr-RS218 ∆cjr-RS218 harboring pCL1920 This study
 CjrA-RS218 ∆cjr-RS218 harboring pCL1920-cjrA This study
 CjrB-RS218 ∆cjr-RS218 harboring pCL1920-cjrB This study
 CjrC-RS218 ∆cjr-RS218 harboring pCL1920-cjrC This study
 SenB-RS218 ∆cjr-RS218 harboring pCL1920-senB This study
Plasmids
 pCL1920 Low-copy-number plasmid [19]
 pCL1920-cjrABC-senB pCL1920 harboring cjrABC-senB, which is under the control of the lac promoter on the plasmid This study
 pCL1920-cjrA pCL1920 harboring cjrA with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. This study
 pCL1920-cjrB pCL1920 harboring cjrB with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. This study
 pCL1920-cjrC pCL1920 harboring cjrC with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. This study
 pCL1920-senB pCL1920 harboring senB with a His6-tagged 3′-end, which is under the control of the lac promoter on the plasmid. This study