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Table 2 Post-Antibiotic Effect (PAE) and Score of Post-Antibiotic Effect-associated Permeabilization (S-PAEP) induced by P4–9

From: A synthetic peptide sensitizes multi-drug resistant Pseudomonas aeruginosa to antibiotics for more than two hours and permeabilizes its envelope for twenty hours

Strain PAEa(h) PAEPb (h) Antibiotic (MIC)c Final concentration in μg/mL (times x MIC) S-PAEPd (h)
0 1 2
Ps4 1.45 + 0.02 2–3 NOV (512) 64 (1/8) 4.8 + 0.1 2.7 + 0.05 0.45 + 0.01
  2–3 FOS (512) 64 (1/8) 2.27 + 0.05 2.12 + 0.04 1.60 + 0.03
  2–3 RIF (16) 2 (1/8) 0.6 + 0.01 0.6 + 0.01 0.25 + 0.01
  2–3 ERY (128) 16 (1/8) 1.04 + 0.02 0.82 + 0.02 0.78 + 0.02
  2–3 CAZ (64) 16 (1/4) 14.24 + 0.3 14.24 + 0.3 1.56 + 0.03
  2–3 CAZ (64) 4 (1/16) 2.0 + 0.04 1.25 + 0.03 0.1 + 0.01
  2–3 CEF (1024) 512 (1/2) 0.8 + 0.02 0.7 + 0.01 0.7 + 0.01
  n.fe CIP (2) 0.25 (1/8) 0.7 + 0.01 < 0.25 < 0.25
PAO1 1.9 + 0.03 2–3 NOV (1024) 64 (1/16) 7.81 + 0.16 2.58 + 0.05 0.87 + 0.02
LC1–6 0.75 + 0.04 2–3 NOV (8192) 512 (1/16) 7.62 + 0.15 4.29 + 0.1 0.87 + 0.02
PAΔADD 1.2 + 0.026 2–3 CAZ (64) 16 (1/4) 4.27 + 0.1 3.42 + 0.07 3.32 + 0.07
h2Dh3
  1. a Post-antibiotic effect assessed by turdidimetry (Bioscreen). PAEs y S-PAEPs values are the average of three and two independent experiments, respectively, each of them performed in triplicate. Differences were analyzed by Kruskal–Wallis test followed by Mann–Whitney’s U test
  2. b Duration of Post-Antibiotic Effect-associated Permeabilization
  3. c NOV, novobiocin; FOS, fosfomycin; RIF, rifampin; ERY, erythromycin; CAZ, ceftazidime; CEF, cephalothin; CIP, ciprofloxacin. MICs (μg/mL) are in parenthesis
  4. d Score of Post-Antibiotic Effect-associated Permeabilization. Delay in h caused by exposure to a sub-MIC concentration of an antibiotic added at the indicated time (0 h, 1 h, 2 h) after the beginning of growth in peptide-free medium. S-PAEP was assessed by turdidimetry (Bioscreen)
  5. e Determination was not feasible due to resistance to antibiotic