|
Strain
|
PAEa(h)
|
PAEPb (h)
|
Antibiotic (MIC)c
|
Final concentration in μg/mL (times x MIC)
|
S-PAEPd (h)
|
|---|
|
0
|
1
|
2
|
|---|
|
Ps4
|
1.45 + 0.02
|
2–3
|
NOV (512)
|
64 (1/8)
|
4.8 + 0.1
|
2.7 + 0.05
|
0.45 + 0.01
|
| |
2–3
|
FOS (512)
|
64 (1/8)
|
2.27 + 0.05
|
2.12 + 0.04
|
1.60 + 0.03
|
| |
2–3
|
RIF (16)
|
2 (1/8)
|
0.6 + 0.01
|
0.6 + 0.01
|
0.25 + 0.01
|
| |
2–3
|
ERY (128)
|
16 (1/8)
|
1.04 + 0.02
|
0.82 + 0.02
|
0.78 + 0.02
|
| |
2–3
|
CAZ (64)
|
16 (1/4)
|
14.24 + 0.3
|
14.24 + 0.3
|
1.56 + 0.03
|
| |
2–3
|
CAZ (64)
|
4 (1/16)
|
2.0 + 0.04
|
1.25 + 0.03
|
0.1 + 0.01
|
| |
2–3
|
CEF (1024)
|
512 (1/2)
|
0.8 + 0.02
|
0.7 + 0.01
|
0.7 + 0.01
|
| |
n.fe
|
CIP (2)
|
0.25 (1/8)
|
0.7 + 0.01
|
< 0.25
|
< 0.25
|
|
PAO1
|
1.9 + 0.03
|
2–3
|
NOV (1024)
|
64 (1/16)
|
7.81 + 0.16
|
2.58 + 0.05
|
0.87 + 0.02
|
|
LC1–6
|
0.75 + 0.04
|
2–3
|
NOV (8192)
|
512 (1/16)
|
7.62 + 0.15
|
4.29 + 0.1
|
0.87 + 0.02
|
|
PAΔADD
|
1.2 + 0.026
|
2–3
|
CAZ (64)
|
16 (1/4)
|
4.27 + 0.1
|
3.42 + 0.07
|
3.32 + 0.07
|
|
h2Dh3
|
- a Post-antibiotic effect assessed by turdidimetry (Bioscreen). PAEs y S-PAEPs values are the average of three and two independent experiments, respectively, each of them performed in triplicate. Differences were analyzed by Kruskal–Wallis test followed by Mann–Whitney’s U test
- b Duration of Post-Antibiotic Effect-associated Permeabilization
- c NOV, novobiocin; FOS, fosfomycin; RIF, rifampin; ERY, erythromycin; CAZ, ceftazidime; CEF, cephalothin; CIP, ciprofloxacin. MICs (μg/mL) are in parenthesis
- d Score of Post-Antibiotic Effect-associated Permeabilization. Delay in h caused by exposure to a sub-MIC concentration of an antibiotic added at the indicated time (0 h, 1 h, 2 h) after the beginning of growth in peptide-free medium. S-PAEP was assessed by turdidimetry (Bioscreen)
- e Determination was not feasible due to resistance to antibiotic
