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Table 3 Post-Antibiotic Effect (PAE) and Score of Post-Antibiotic Effect-associated Permeabilization (S-PAEP) induced by antibiotics on Ps4

From: A synthetic peptide sensitizes multi-drug resistant Pseudomonas aeruginosa to antibiotics for more than two hours and permeabilizes its envelope for twenty hours

Agent used as pre-treatmenta MIC/MBC (μg/mL) Final concentration (μg/mL) PAEb (h) PAEPc (h) Snov-PAEPd (h)
0 1 2
GEN 2/2 4 0.75 + 0,01 0 < 0.25 < 0.25 < 0.25
CIP 2/2 4 1.5 + 0,06 0 < 0.25 < 0.25 < 0.25
IPM 2/2 4 0 0 < 0.25 < 0.25 < 0.25
PMB 1/1 2 0.1 + 0,02 2–3 3.2 + 0.06 2.7 + 0.05 1.1 + 0.03
  1. a GEN, gentamicin; CIP, ciprofloxacin; IPM, imipenem; PMB, polymyxin B
  2. b Post-antibiotic effect assessed by turdidimetry (Bioscreen)
  3. c Duration of Post-Antibiotic Effect-associated Permeabilization. PAEs y S-PAEPs values are the average of two independent experiments performed in triplicate. Differences were analyzed by Kruskal–Wallis test followed by Mann–Whitney’s U test
  4. d Score of Post-Antibiotic Effect-associated Permeabilization. Delay in h caused by exposure to a sub-MIC concentration of novobiocin (1/8 x MIC) added at the indicated time (0 h, 1 h, 2 h) after the beginning of growth in peptide-free medium. S-PAEP was assessed by turdidimetry (Bioscreen)