Fig. 3

Chemical analysis of PS–protein conjugates. a SDS-PAGE of S. Typhimurium PS–BSA (6a) and PS–OVA (6b) conjugates using Coomassie brilliant blue. Lanes 1–4 are PS–protein conjugate in 4, 10, 20 and 40 μg, respectively. Lane 5 is unmodified protein (2 μg), and lane 6 is unmodified LFPS (40 μg). b FPLC diagram of PS − A–B–OVA (7b) conjugate, which was prepared from S. Typhimurium fraction-1 LFPS. Column: Superdex-200 pg; eluent: PBS at a flow rate of 1 mL/min. a and b are the diagrams using RI and UV-vis (λ = 280 nm) detectors, respectively