Fig. 4
From: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide
hTLR5 activation by PS–linker–FliC conjugates 7c, 8b and 9b. Unmodified FliC was used to prepare 7c, whereas site-selective azido-modified FliC was used to prepare 8b and 9b. The concentration is estimated by the sum of the mass of carbohydrates and proteins. Native FliC is positive control, and 1× PBS is negative control. ** p < 0.01 and *** p < 0.001. The data are presented as mean ± standard deviation (n = 3). A comparison of paired samples was performed by using Student’s t-test