Fig. 5

Endpoint titers of immune serum IgG specific to S. Typhimurium LPS (A–C) or FliC (D). BALB/c mouse (n = 5 for each group) was immunized: (a) using PS–QXO–BSA (6a) or PS–QXO–OVA (6b) at 2.5 μg dosage. Freund’s complete adjuvant was injected on week 0, and the Freund’s incomplete adjuvant was injected on weeks 2, 4 and 6. (b) using PS–A − B − BSA (7a) or PS–A − B − OVA (7b) prepared from fraction-1 LFPS at 2.5 μg or 5.0 μg dosage, and (c) using PS–A − B − FliC (7c), PS − A − B − C − FliC (8b) or PS − A − B − K3G5 − FliC (9b) at 2.5 μg dosage. The data were derived from two independent experiments. PBS was negative control. The cut-off value was defined as two-fold of the absorbance reading of negative control. * p < 0.05, ** p < 0.01 and *** p < 0.001. The data are presented as mean ± standard deviation (n = 5). A comparison of paired samples was performed by using Paired t-test