Skip to main content

Table 1 Carbohydrate-to-protein molar ratio and estimated conjugation number of the synthesized PS − protein conjugates

From: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide

Entry PS–protein conjugatea PS
μg/mL (μM)b
Protein
μg/mL (μM)c
Conjugation numberd
1 7a (BSA)e 168.7 (1.97) 62.4 (0.95) 2–3 (2.1)d
2 7a (BSA)f 98.2 (4.27) 13.2 (0.20) 20–23 (21.8)d
3 7b (OVA)e 342.2 (3.99) 123.0 (2.80) 1–2 (1.4)d
4 7c (FliC)f, g 168.4 (7.32) 24.0 (0.47) 14–17 (15.6)d
5 8a (BSA)f 94.1 (4.09) 13.8 (0.21) 18–21 (19.5)d
6 8b (FliC)f, h 235.5 (10.24) 83.1 (1.63) 5–7 (6.3)d
7 9a (BSA)f 95.4 (4.15) 15.2 (0.23) 17–19 (18.0)d
8 9b (FliC)f, h 221.0 (9.61) 96.8 (1.90) 4–6 (5.1)d
  1. a PS−protein conjugates prepared from S. Typhimurium LFPS, and isolated by FPLC−SEC
  2. b Data derived from PSA assay by calibration of the absorbance at 490 nm
  3. c Data derived from BCA assay by calibration of the absorbance at 562 nm based on the molecular weights of OVA (44 kDa), BSA (66 kDa) and FliC (51 kDa)
  4. d Estimated number of LFPS on each PS–protein conjugate. Data in parenthesis are calculated from the molar ratio of PS over protein
  5. e The PS−protein conjugate was prepared from fraction-1 LFPS with a median molecular weight of 85.8 kDa
  6. f The PS−protein conjugate was prepared from unfractionated LFPS with average molecular weight of 23 kDa
  7. g Conjugate 7c was prepared by the coupling reaction of PS−A−B/Np with FliC
  8. h The azido-modified FliC was prepared by site-selective method, and used in synthesis of 8b and 9b