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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Clinically relevant radioresistant rhabdomyosarcoma cell lines: functional, molecular and immune-related characterization

Fig. 1

Development of clinically relevant radio-resistant cell line. a Representation of the radiation schedule used and the related radiobiological parameters. Growing RD and RH30 cells at 80% of confluence were irradiated with the dose of 6 Gy. 24 h after irradiation, 30% of irradiated cells were re-seeded and the next irradiation repeated when a confluence of 80% was reached again, this for 6 times to get a final equivalent dose (EQD2) to that reached with conventional fractionation of 66 Gy into daily dose of 2 Gy. b Upper Panel. Clonogenic assay of the parental (PR) and clinically relevant (RR) RD (Left Panel) and RH30 (Right Panel) lines with increasing dose of irradiation (2, 4, 6, 8 Gy). Data are expressed as relative optical density vs. non-irradiated cells, taken as 1. Representative crystal violet stained cultures 14 days after irradiation. c Percent of plating efficiency. Results represent the mean values of four independent experiments ± SD. Statistical significance: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared RMS-PR vs. RMS-RR

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