Fig. 2

Onco-phenotype characterization of clinically relevant radio-resistant cell line. a Panels show the ability of RMS-PR and -RR to attach and spread (Left Panel) and proliferate (Right Panel) on a fibronectin coated plate. Data of attachment assay are expressed as fold of increase vs. non-irradiated cells, taken as 1. b Wound healing experiments in RMS-PR and RMS-RR cells. A scratch was made at time 0 and maintained or not for 16 h. The dotted lines represent the edges of the wound. Photographs (Left Panel) were taken under light microscope (10x magnification). The migration index was plotted in bar graphs as the % of wound area (Right Panel). c Matrigel invasion assay. Cells were allowed to invade for 24 h in serum-free medium. Pictures shown are the most representative from three independent experiments. The graph represents absorbance at 595 nm after incubation of the membranes with deoxycholic acid. Results represent the mean values of four independent experiments ± SD. Statistical significance: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared RMS-RR vs. RMS-PR. d Representative microphotographs of RMS-PR and RMS-RR cells after 14 days of incubation in stem cell medium