Fig. 3

RMS-PR and -RR cells differently trigger anti-oxidant and DNA repair responsiveness after irradiation. a Mitochondrial superoxide anion production was assessed by MitoSox Red staining, 10 min (0.1), 30 min (0.5), 12 or 24 h after RT in RMS-PR and RMS-RR cells. b Gene expression of antioxidant enzymes nuclear factor erythroid 2-related factor (NRF2), superoxide dismutase (SOD-2), catalase (CAT) and glutathione peroxidase (GPx)-4 was investigated by real-time PCR, 12 h after RT. The gene expression was referenced to the ratio of the value of interest and basal conditions. The value of basal conditions was reported equal to 1. c Cell lysates from RMS-PR and RMS-RR cells untreated (−) or treated (+) with 6 Gy of irradiation collected 12 h after RT, were analyzed by immunoblotting with specific antibodies for indicated proteins; α-Tubulin expression shows the loading of samples. Western blot showed are representative of three different experiments. Statistical analyses: *p < 0.05, **p < 0.01, ***p < 0.001 RMS-RR NO RT vs. RMS-PR NO RT, ^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 RMS-PR RT vs. RMS-PR NO RT and RMS-RR RT vs. RMS-RR NO RT, ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 RMS-RR RT vs. RMS-PR RT.