Fig. 4

Irradiation differently changes cell cycle distribution in RMS-PR and -RR cells. a Effect of irradiation with 6 Gy on cell number of RMS-PR and RMS-RR. b FACS analysis performed on RMS-PR and RMS-RR cells after 24, 48 and 72 h from irradiation with 6 Gy. Representative of three different experiments. Results are representative of three different experiments performed in triplicate. c Cell lysates from RMS-PR and RMS-RR cells untreated (−) or treated (+) with 6 Gy of irradiation collected 12 h after RT, were analyzed by immunoblotting with specific antibodies for indicated proteins; α-Tubulin expression shows the loading of samples. Western blot showed are representative of three different experiments. Statistical analyses: *p < 0.05, **p < 0.01, ***p < 0.001 RMS-RR NO RT vs. RMS-PR NO RT, ^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 RMS-PR RT vs. RMS-PR NO RT and RMS-RR RT vs. RMS-RR NO RT, ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 RMS-RR RT vs. RMS-PR RT.