Fig. 8

hG-CSF gene therapy effect on autophagy. a, b The expression level of the autophagy initiator protein; Beclin 1 is demonstrated a 4 and b 7-days post BCAO. c, d expression levels of the autophagosome marker, c LC3-ll and the autophagic substrate, d p62, 7 -days post BCAO. Respective densitometry of Beclin 1, LC3-ll and p62 are also shown. Statistical analysis was performed by One Way ANOVA, with Tukey as post hoc test and expressed as means ± S.E.M; *p < 0.05 or **p < 0.001 versus Sham group while ^#p < 0.05 or ^##p < 0.001 versus AAV-CMV-GFP- treated group (Sham n = 5, AAV-CMV-GFP n = 6, AAV-CMV-GFP n = 6)