Fig. 6

Electrophysiology analysis and behavior of Klhl17^+/– mice. a, b Klhl17^+/– mice exhibit increased mEPSC amplitudes. The mEPSCs of cultured hippocampal neurons were recorded at 18–19 DIV. Klhl17^+/– neurons show higher mEPSC amplitudes, but not mEPSC frequencies. a Representative mEPSC patterns. b Quantification of mEPSC frequency and amplitude. c–e Klhl17^+/– mice exhibit hyperactivity in an open field (c) and lower social interaction in the reciprocal social interaction test (e), but presented no difference to wild-type mice in the elevated plus maze (d). a, b Samples were randomly collected from four independent experiments without knowing the genotypes. N the number of analyzed neurons. Data represent the mean plus SEM. *P < 0.05; **P < 0.01; ns not significant. Scale bar: a 10 pA, 1 s. Unpaired t-test (b–e)