Fig. 1

Siglec-E deficiency promotes foam cells and necrotic core in atherosclerotic lesion in apoE^−/− mice. ApoE^−/− and apoE^−/−/SE^−/− mice were placed on HFD for 3 months. Heart and aortic tissues were harvested (a–d). a The representative images of oil-red dye (ORD) stained aortic tissues. b The percentages of ORD stained areas in both groups of mice were quantified. c The representative images of Trichrome stained aortic root sections of male mice. d The lesion sizes and the percentages of lipid cores in the aortic roots were quantified. e Peritoneal macrophages isolated from HFD-fed apoE^−/− and apoE^−/−/SE^−/− mice were examined by ORD staining. f The mRNA expression levels of indicated cytokines in macrophages were examined by quantitative RT-PCR