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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Siglec-E retards atherosclerosis by inhibiting CD36-mediated foam cell formation

Fig. 2

Siglec-E deficiency facilitates modified LDL uptake without affecting cholesterol efflux. Peritoneal macrophages were isolated from WT and SE−/− mice. a Macrophages were incubated with 50 µg/ml of oxLDL or acLDL as indicated for 24 h in culture. The accumulation of cholesterols was examined by ORD staining. b The quantitative results of ORD intensities. Data shown are the mean ± SE of 3 independent experiments. *P < 0.02 vs WT. c Macrophages were incubated with 10 μg/ml of Dil-oxLDL or Dil-acLDL at 37 °C for indicated times. The amounts of uptake in each time point were quantified. Data shown are mean ± SE of 4 independent experiments. *P < 0.03 vs WT. d The representative images showing Dil-oxLDL or Dil-acLDL uptake after 1 h incubation. e Macrophages preloaded with fluorescently-labeled cholesterol analogue were incubated without (negative) or with indicated agents for 6 h in culture. The percentage of fluorescence intensity in the medium was calculated. Results shown are the mean ± SE of 3 mice in each genotype

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