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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Siglec-E retards atherosclerosis by inhibiting CD36-mediated foam cell formation

Fig. 5

Sialic acid is involved in Siglec-E-mediated inhibition of oxLDL uptake by CD36. a HEK293T cells transfected with indicated vectors for 48 h were treated without or with sialidase (0.1 unit/ml) for 30 min at 37 °C, followed by incubation with Dil-oxLDL (5 μg/ml) at 37 °C for 1 h. The intracellular accumulation of Dil-oxLDL was examined by confocal microscopy. b The quantitative results of Dil-oxLDL uptake in various groups of cells. Data shown are the mean ± SE of 3 independent experiments. *P < 0.05 vs cells overexpressing Flag-CD36 alone. c Peritoneal macrophages were pretreated without or with sialidase, followed by incubation with Dil-oxLDL as described above. Dil-oxLDL uptake was examined by confocal microscopy. d The quantitative results of (c). Data shown are the mean ± SE of 3 independent experiments. *P < 0.01 vs untreated cells. e HEK293T cells transfected with indicated vectors were treated without or with oxLDL (50 μg/ml) for 30 min at 37 °C. Cell lysates were subjected to immunoprecipitation with anti-HA tag affinity resin. The immunoprecipitates were then analyzed by Western blotting with anti-phosphotyrosine and anti-HA antibodies. The relative quantitative results were shown under the blot. f WT peritoneal macrophages were pretreated with or without sialidase, followed by incubation without or with oxLDL as described above. Cell lysates were prepared and immunoprecipitation was performed with anti-Siglec-E antibody. The immunoprecipitates were then analyzed by Western blotting with anti-phosphotyrosine and anti-Siglec-E antibodies. The relative quantitative results were shown under the blot

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