Skip to main content
Fig. 1 | Journal of Biomedical Science

Fig. 1

From: O6-methylguanine-DNA methyltransferase modulates cisplatin-induced DNA double-strand breaks by targeting the homologous recombination pathway in nasopharyngeal carcinoma

Fig. 1

MGMT inhibition suppressed CDDP-induced RAD51 expression in NPC cells. a A heat map showing the expression changes of DNA repair genes with statistical significance, by using RT2 Profiler PCR Array, in HONE-1 cells with or without O6BG treatment, respectively. After HONE-1 cells were treated with O6BG (120 μM) for 8 h, the extracted mRNA was subjected to gene expression analyses. b HONE-1 and c TW01 cells were treated with O6BG at indicated concentrations for 8 h. d HONE-1 and e TW01 cells were treated with O6BG (60 μM), CDDP (10 µM), or a combination treatment for 8 h. The IC50 concentration of O6BG in both HONE-1 and TW01 cells was 120 µM. After the indicated treatment, cell lysates were analyzed using Western blotting. Fold changes in protein levels listed under each blot were normalized to the levels of the actin control. Following single-drug or combination treatment as indicated in d and e, the mRNA levels of f HONE-1 and g TW01cells were quantified using qPCR. Representative results of at least three independent experiments are presented. Bar values are presented as mean ± SD of at least three independent experiments. *P < 0.05

Back to article page