Fig. 2

Cell cycle arrest in late-S and G2/M in MPA-treated cells. DMSC were treated with MPA for 48 h and then incubated with propidium iodide (PI) and RNase for 15 min. a Fluorescence histograms obtained by flow cytometry analysis of stained cells: Y-axis gives the number of cells, and the X-axis gives PI fluorescence intensity, which is proportional to DNA content. Cells treated with MPA tended to be retained in the late S phase (arrow) as well as being arrested in G2/M (representative image of three experiments). b Comparison of the percentages of cells in gated areas corresponding to G0/G1 and G2/M in untreated and MPA-treated cells (n = 3) (**P ≥ 0.01)