Table 1 The primer sequences for constructing FKBP10 domain deletion mutants
From: FKBP10 promotes proliferation of glioma cells via activating AKT-CREB-PCNA axis
FKBP10 mutants | Forward | Reverse |
|---|---|---|
∆1 (aa1–150) | AACAAGGAAGACACCGTGCAGGTGAGCACAT | CATAGAATTCGAAGCTTGAGCTCGAGATC |
∆2 (aa174–262) | AACCCGAAGGACGCTGTCCAGCTAGAGAC | GTCCTGGACCATGCGGGGGCAGTG |
∆3 (aa286–374) | AACCCTGCGGATGTGGTGGAAATCAGGACAC | CCCGGCCCCGGCTCTGCGGACA |
∆4 (aa399–486) | GATGGGCTGCCCACAGGCTACCTGTTTG | AAGCTTGGTGGTCTCATTGCAGGTCTCAGATGG |
∆5 (497–582) | GGATCCACCGGATCTAGATAACTGATCATAAT | CACAAACAGGTAGCCTGTGGGCAGCCC |