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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: The ESCRT-III molecules regulate the apical targeting of bile salt export pump

Fig. 1

Aberrant subapical BSEP compartments in cholestatic human livers are CHMP5 positive. a An illustration of the post-Golgi trafficking of BSEP in hepatocytes. De novo synthesized BSEP (green) targets the subapical compartments (SACs) from the trans-Golgi and constitutively cycles between SACs and the apical/canalicular membrane (blue) of hepatocytes. b–f Aberrant subapical BSEP compartments in cholestatic human livers are associated with CHMP5 co-expression. Immunofluorescence staining demonstrated BSEP and the ESCRT-III CHMP5 in the subcellular compartments of hepatocytes in human liver samples from b a representative infant control; c a patient with cholestasis in infancy harboring heterozygous BSEP-R487H mutation (BSEP-R487H/ +); d a PFIC2 infant with BSEP-W493X/G1004D mutations; e a diseased child with transient cholestasis; and f a representative patient with neonatal hepatitis. Cryosection of human livers samples were stained for BSEP (green) and CHMP5 (red). The liver nuclei were stained with DAPI. The arrowhead indicates the apical/canalicular membrane of hepatocytes; arrows indicate the aberrant BSEP vesicles in the cytoplasm of cholestatic livers. Notably, two different patterns of BSEP and CHMP5 were observed in the sample of transient cholestasis e and referred to Additional file 1: Figure S1

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