Fig. 5

l-Carnitine treatment increased mitochndria activity and the type I and type II myosin light chain expression of TPM3(E151G) larva. Mitochondrial respiration of TPM3 transgenic zebrafish via seahorse. a Schematic and oxygen consumption rate (OCR). OCR was measured under basal conditions followed by the sequential addition of oligomycin (0.5 μM), Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP) (4 μM), rotenone (1 μM), or antimycin A (1 μM). b Basal respiration, c ATP production, and d proton leak of OCR measurement. e NADH-TR stains of TPM3(WT), TPM3(E151G) and one month of L-carnitine treated TPM3(E151G) transgenic zebrafish at 400X, scale bar is 50 µm. f the expression level of myh7 and g myhz2 from TPM3 transgenic fish (F3) treated with l-carnitine from 28 hpf to 7 dpf cpmpared to TPM3(WT). Statistical significance was determined using a t-test, *0.01 < P ≤ 0.05; **0.001 < P ≤ 0.01; ***0.0001 < P ≤ 0.001; ****P ≤ 0.0001