Table 1 Sequences of qPCR primers
Gene | Primer | Sequence 5′ – 3′ | Probe no. |
|---|---|---|---|
ACTA2 | Forward | 5′- CCAGCACCATGAAGATCAAG − 3′ | 58 |
ACTA2 | Reverse | 5′- TCCACATCTGCTGGAAGGTA − 3′ | |
COL1A1 | Forward | 5′- CATGTTCAGCTTTGTGGACCT − 3′ | 15 |
COL1A1 | Reverse | 5′- GCAGCTGACTTCAGGGATGT − 3′ | |
CTGF | Forward | 5′- TGACCTGGAGGAAAACATTAAGA − 3′ | 71 |
CTGF | Reverse | 5′- AGCCCTGTATGTCTTCACACTG − 3′ | |
ET-1 | Forward | 5′- TCCTTGATGGACAAGGAGTGT − 3′ | 29 |
ET-1 | Reverse | 5′- CCCAATCCATACGGTACGAC − 3′ | |
GAPDH | Forward | 5′- GCCAAAAGGGTCATCATCTC − 3′ | 29 |
GAPDH | Reverse | 5′- CACACCCATCACAAACATGG −3′ |