Skip to main content
Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Modulation of human platelet activation and in vivo vascular thrombosis by columbianadin: regulation by integrin αIIbβ3 inside-out but not outside-in signals

Fig. 2

Effectiveness of columbianadin (CBN) on surface P-selectin expression, ATP releases, and relative [Ca2+]i mobilization in human platelets. Washed platelets (3.6 × 108 cells/mL) were preincubated with the solvent control (0.1% DMSO) or CBN (60 and 80 μM), followed by the addition of collagen (1 μg/mL) or arachidonic acid (AA; 60 μM) to trigger (a) surface P-selectin expression (resting control, red line; collagen-activated, black line; CBN 60 μM, blue line; CBN 80 μM, green line), (b) ATP release (AU; arbitrary unit), and (c) relative [Ca2+]i mobilization, as described in the Materials and methods section. The corresponding statistical data are presented in the right panel of each figure. Data are presented as means ± SEM (n = 4). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the resting control; ##p < 0.01 and ###p < 0.001, compared with the 0.1% DMSO-treated group

Back to article page