Fig. 3

Regulatory effects of columbianadin (CBN) on PLCγ2/PKC and Akt phosphorylation in platelets. Washed platelets were preincubated with a solvent control (0.1% DMSO) or CBN (60 and 80 μM) and subsequently treated with collagen (1 μg/mL), PDBu (150 nM) or arachidonic acid (AA; 60 μM) to trigger either (a) PLCγ2, (b) PKC (p47) activation or (c) platelet aggregation, and (d) Akt phosphorylation. Profiles in (c) are representative of four independent experiments. Data are presented as the means ± SEM (n = 4). ^**p < 0.01 and ^***p < 0.001, compared with the resting platelets; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001, compared with the 0.1% DMSO-treated group