Fig. 8
From: “Point of no return” in unilateral renal ischemia reperfusion injury in mice
Course of cell death after ischemia for 35 min and reperfusion times between 1 h and 5 weeks. a: TUNEL staining of ischemic kidney sections after ischemia for 35 min and assessment at different time points until 5 weeks revealed highest intensity after 6 h with rapid decline to a constant level between 48 h and 35 d. b: Images of Lotus tetragonolobus lectin (red) and TUNEL (green) co-stained sections (100x magnification) of renal cortices of kidneys undergone unilateral ischemia for 35 min at different reperfusion times. The weakening signal for Lotus tetragonolobus lectin indicates progressing tubule atrophy over time and does not allow to define the dying cell type after 72 h and 35 d, whereas the majority of TUNEL positivity derives from proximal tubule epithelial cells after 6 h. Displayed are means of 3 sections analyzed using ImageJ with s.e.m