Fig. 4

Expression and location of elastolytic enzymes in the aorta and PVAT. Expression of genes for cathepsins and MMPs in the a aorta (n = 7, 5, and 6 for control, ob/ob, and ob/ob + Piog groups respectively) and b PVAT (n = 3, 5, and 6 for control, ob/ob, ob/ob + Piog groups respectively). mRNA amount is expressed relative to the average expression in control mice. *P < 0.05, **P < 0.01, and ***P < 0.001. ND, not detectable. c Immunofluorescence staining for CTSS (green), MMP-12 (red) and F4/80 (blue) in the thoracic aorta and PVAT. The DAPI nuclear counterstain appears light blue. Magnification of the square in (c) is shown in (d). The while arrow indicates the location of elastic fiber break with increased signals of MMP-12 and F4/80. e Plasma levels of CTSS, MMP-12, and MCP-1 by ELISA (n = 7, 5, and 6 for control, ob/ob, and ob/ob + Piog groups respectively). All data are resulted from 3-month-old male mice. Original magnification × 400 for (c) and × 1200 for (d)