Fig. 6From: PPARγ activation improves the microenvironment of perivascular adipose tissue and attenuates aortic stiffening in obesityBinding of PPARγ in Ctss and Mmp12. ChIP assay in Raw 264.7 and 3T3-L1 undifferentiated cells treated with pioglitazone. a, b The schematic representation shows the location of primers flanking putative PPARγ-binding sites (grey rectangles) in Ctss and Mmp12. Sequences containing the potential PPARγ-binding sites in Ctss and Mmp12 loci were amplified by real-time polymerase chain reaction in (c, d) Raw 264.7 and (e, f) 3T3-L1 undifferentiated cells. Data were collected from three independent experimentsBack to article page