Fig. 3
From: NKX6-1 mediates cancer stem-like properties and regulates sonic hedgehog signaling in leiomyosarcoma
NKX6-1 promotes malignancy in LMS cells. Overexpression of NKX6-1 in MES-SA LMS cells increases proliferation and colony formation. Knockdown of NKX6-1 in MES-SA/DX5 cells, by contrast, decreases proliferation. a A cell proliferation assay was performed using the MTS assay. b Colony formation ability was assessed using the AIG assay. NKX6-1-overexpressing MES-SA cells c and NKX6-1 knockdown MES-SA/DX5 cells d were evaluated for resistance to cisplatin, gemcitabine, phyxol, and doxorubicin. The concentrations of cisplatin, gemcitabine, phyxol, and doxorubicin in c are 0.33, 0.01, 0.005 and 7.5 μM, respectively (the IC50 in MES-SA-VC cells). The concentrations of cisplatin, gemcitabine, phyxol, and doxorubicin in d are 0.5, 0.05, 0.0125 and 30 μM, respectively (the IC50 in MES-SA/DX5-Scramble cells). e In vivo tumor growth of NKX6-1-overexpressing MES-SA cells. *p < 0.05