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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Proteomics-based identification of TMED9 is linked to vascular invasion and poor prognoses in patients with hepatocellular carcinoma

Fig. 5

Depletion of TMED9 decreases cell growth and invasion abilities of hepatocellular carcinoma (HCC) cells. a Mahlavu cells were infected with a lentivirus carrying control shRNA (sh-Ctrl) or two TMED9 shRNAs (shTMED9). After 72 h, knockdown efficiencies of the two TMED9 shRNAs were determined by Western blotting. b–d Cell-proliferative, colony-forming, and invasive abilities of TMED9-knockdown Mahlavu cells were respectively determined by Alamar blue cell-viability (b), colony-formation (c), and transwell-invasion d assays. Upper panel of C and left panel of D: representative photomicrographs. Values are presented as the mean ± SD from three independent experiments. ***p < 0.001 compared to the sh-Ctrl group

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