RhoA: a dubious molecule in cardiac pathophysiology

Table 1 GEFs, GAPs, GDIs, and GDFs of RhoA

Group	Regulator name in human (mouse)	Interaction with SMGS	Domains	Receptor activation	References
GEFs	Dbl	Rho/Rac/Cdc42	DH/PH	(Gαq/11)	[56, 136, 174]
	Dbs	Rho, Cdc42	DH/PH	(Gαq/11)	[136, 171]
	LARG-RhoGEF (ARHGEF12)	Rho	DH/PH, PDZ	Gα12/13	[3, 159]
	Lbc (AKAP/ARHGEF13)	Rho	DH/PH	Gα12/13	[3, 183]
	Lfc (ARHGEF2/GEF-H1)	Rho	DH/PH	Gα12/13	[50, 107]
	p63RhoGEF, *GEFT	Rho	DH/PH	Gαq/11	[100, 136]
	p115RhoGEF (ARHGEF1/Lsc)	Rho	DH/PH, RH	Gα12/13	[50, 57, 85]
	PDZ-RhoGEF (ARHGEF11)	Rho	DH/PH, RH, PDZ	Gα12/13	[3, 46]
	Vav1	Rho/Rac/Cdc42	DH/PH, SH2	Src	[28]
	Vav2	Rho	DH/PH, SH2	Src	[145]
Group	Regulator (name)	Interaction with SmGs	Model		Reference
GAPs	Graf	Rho, Cdc42	Chicken embryo (CE) cells		[62]
	Dlc1	Rho/Rac/Cdc42	Tumor cells (liver, HeLa)		[177]
	Myr5	Rho, Cdc42	Isolated GTPases		[129]
	RA-RhoGAP	Rho	Smooth muscle		[184]
	ARAP3	Rho, Arf	Smooth muscle		[184]
	p50RhoGAP	Rho, Rac, Cdc42	Kidney cells (HEK), fibroblasts (COS-7)		[11, 87]
	p190RhoGAP	Rho Rac, Cdc42	Tumor cells (HeLa), fibroblasts (COS-7), neurons		[7, 59, 132]
Group	Regulator (name)	Interaction with SmGs	Model		Reference
GDIs	RhoGDI1 (RhoGDIα)	Rho/Rac/Cdc42	Neurons		[166]
	*RhoGDI2 (RhoGDIβ/D4/ Ly-GDI)	Rho/Rac/Cdc42	Lymphocytes		[92, 144]
	*RhoGDI3 (RhoGDIγ)	RhoB and RhoG (not RhoA and RhoC)	Tumor cells (HeLa)		[180]
Group	Regulator (name)	Interaction with GDIs	Model		Reference
GDFs	ERM	RhoGDIα	Fibroblasts (COS-7)		[160]
	p75NTR	RhoGDI	Neuroblasts (PC12)		[175]
	IΚΚγ/NEMO	RhoGDI	HEK cells (293 T)		[80]

Listed are the GEFs, GAPs, GDIs and GDFs which have been shown to interact with RhoA. For the GEFs the SmG-(sub-) family with which members they interact besides RhoA is listed, as well as the conserved domains which are responsible for the interaction with the SmG and the mechanism of activation. For the GAPs, GDIs and GDFs also the interaction partners and the cell model in which the activity on RhoA has been shown are listed. (* = isoform)