Fig. 6

IGPR-1 is expressed in human melanoma and knockdown of NEDD4 increases IGPR-1 expression. a, b Expression of IGPR-1 in human melanoma. Data extracted from TCGA dataset via cBioportal (http://www.cbioportal.org/). c The volcano plot of IGPR-1 is shown. d Expression of IGPR-1 and NEDD4 in human melanoma cell lines. e A375 melanoma cells were transfected with human NEDD4 shRNA and after selection with puromycin cells were lysed and subjected Western blot analysis using anti-NEDD4, anti-IGPR-1 antibodies. Also shown is the Western blot analysis of A375 cells expressing NEDD4-shRNA with NEDD4-HA. The graph is representative of three independent experiments. P < 0.05