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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Histone deacetylase 7 mediates endothelin-1-induced connective tissue growth factor expression in human lung fibroblasts through p300 and activator protein-1 activation

Fig. 2

ET-1 induces the nuclear translocation of HDAC7 in WI-38 cells. A After ET-1 stimulation for 0, 5, 10, 30, and 60 min, cells were collected. The distribution of HDAC7 was analyzed using a cytoplasmic and nuclear protein extraction kit and Western blotting (n = 4). Lamin A and α-tubulin were loading controls. B After ET-1 stimulation for 0, 5, and 10 min, cells were immunodetected with an antibody specific for HDAC7 (green); nuclei were detected with DAPI (blue) (n = 3). IF staining is described in “Materials and methods.”

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