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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Histone deacetylase 7 mediates endothelin-1-induced connective tissue growth factor expression in human lung fibroblasts through p300 and activator protein-1 activation

Fig. 3

p300 participates in the expression of CTGF caused by ET-1 stimulation in WI-38 cells. A p300 was knocked down by transfection of siRNA for 24 h. ET-1 stimulation proceeded for 2 h. Western blot analysis was conducted to detect the protein level of CTGF. α-Tubulin was the loading control. Bars represent values of the mean ± SEM (n = 4). *p < 0.05, compared with the ET-1 stimulation group. B After transfection with CTGF-Luc (1 μg), Lac Z (1 μg), and p300 siRNA for 24 h, the cells were stimulated by ET-1 for 16 h. The activity of luciferase was measured as described above. Bars represent values of the mean ± SEM (n = 6). *p < 0.05, compared with ET-1 treatment group. C, After ET-1 stimulation for various time intervals, histone acetyltransferase activity was analyzed. Data are presented as mean ± SEM (n = 3). *p < 0.05, compared with the control without ET-1 stimulation as 100%

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