Fig. 4

HDAC7 participated in ET-1-stimulated c-Jun acetylation in WI-38 cells. A Cells were stimulated by ET-1 treatment for 0, 10, 20, 30, and 60 min. After ET-1 treatment, immunoprecipitation was performed with c-Jun antibody. Western blotting was conducted to detect the acetylation of c-Jun. Bars represent values of the mean ± SEM (n = 5). *p < 0.05, compared with the control without ET-1 treatment. After transfection with HDAC7 (B) or p300 siRNA (C) for 24 h, cells were stimulated by ET-1 treatment for 20 min. Immunoprecipitation and Western blotting were conducted to detect the acetylation of c-Jun. Bars represent values of the mean ± SEM (n = 4) *p < 0.05, compared with the ET-1 treatment group