Fig. 5

Involvement of HDAC7 and p300 in ET-1-stimulated AP-1 activation in WI-38 cells. A After transfection with AP-1-Luc (1 μg), Lac Z (1 μg), and HDAC7 siRNA for 24 h, cells were treated with ET-1 for 16 h. Luciferase activity was evaluated as described in “Materials and methods.” Bars represent values of the mean ± SEM (n = 3). *p < 0.05, compared with ET-1 treatment. B After transfection with AP-1-Luc (1 μg), Lac Z (1 μg), and p300 siRNA for 24 h, the cells were treated with ET-1 for 16 h. Luciferase activity was measured as described in “Materials and methods.” Bars represent values of the mean ± SEM (n = 3). *p < 0.05, compared with ET-1 treatment