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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Histone deacetylase 7 mediates endothelin-1-induced connective tissue growth factor expression in human lung fibroblasts through p300 and activator protein-1 activation

Fig. 6

ET-1 promotes the complex formation of HDAC7, p300, and AP-1 in WI-38 cells. After ET-1 (10 nM) stimulation for 10 min, cells were collected and then immunoprecipitated with antibodies specific for c-Jun (A), HDAC7 (B), or p300 (C). Samples were analyzed using Western blotting. Equal loading in each lane is shown by the similar intensities of c-Jun, HDAC7, or p300 (n = 3). D After transfection with HDAC7 siRNA for 24 h, cells were stimulated by ET-1 treatment for 10 min. Immunoprecipitation and Western blotting were conducted to detect the protein–protein interaction between c-Jun and p300 (n = 4). E, After transfection with p300 siRNA for 24 h, cells were stimulated by ET-1 treatment for 10 min. Immunoprecipitation and Western blotting were conducted to detect the protein–protein interaction between c-Jun and HDAC7 (n = 4). F Schematic of the 550-bp ChIP primer located on the CTGF promoter. After transfection with HDAC7 siRNA for 24 h, cells were stimulated by ET-1 treatment for 20 min, and ChIP assay was conducted as described in “Materials and methods.” Equal amounts of each PCR were confirmed by the product for input. Nonimmune IgG was used as a negative control (n = 4)

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