Fig. 7

HDAC7 expression is elevated in the lung of ovalbumin (OVA)-induced airway fibrosis in mice. A Lung from PBS- or OVA-treated mice was homogenized, and the expression of HDAC7 was analyzed through Western blotting. α-Tubulin was the loading control. B Statistical analysis of the results of Western blotting. Each dot represents an individual mouse, and horizontal lines indicate mean values (n = 6). *p < 0.05, compared with the PBS group. C Paraffin sections of lung tissue from the PBS- or OVA-treated mice were stained for α-SMA (red), HDAC7 (green), and nuclei (blue). The red area represents α-SMA-positive cells, the green area represents HDAC7-positive cells, and the yellow area represents the colocation of α-SMA and HDAC7. The epithelial cell layer is labeled by a white arrow. The subepithelial layer is labeled by a white arrowhead. The IF staining and microscope analysis are described in “Materials and methods.” Bar, 100 μm