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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: USP9X promotes apoptosis in cholangiocarcinoma by modulation expression of KIF1Bβ via deubiquitinating EGLN3

Fig. 3

USP9X interacts with EGLN3 and regulates its expressing level. A Lysates from RBE and HUCCT cells were immunoprecipitated with control IgG, an anti- USP9X or an anti-EGLN3 antibody, followed by immunoblotting analysis. B Lysates from HEK293T cells transiently expressed Flag-USP9X and HA-EGLN3 were immunoprecipitated with anti-Flag or an anti-HA antibody, followed by immunoblotting analysis. RBE (C) and HUCCT (D) cells stably expressing shNC, shUSP9X #1 and shUSP9X #2 were subjected to analyzed by immunoblotting using indicated antibody. RBE (E) and HUCCT (F) Cells stably expressing pCDH and Flag-USP9X were analyzed by immunoblotting using indicated antibody

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Journal of Biomedical Science

ISSN: 1423-0127