Skip to main content
Fig. 7 | Journal of Biomedical Science

Fig. 7

From: USP9X promotes apoptosis in cholangiocarcinoma by modulation expression of KIF1Bβ via deubiquitinating EGLN3

Fig. 7

USP9X promotes apoptosis by targeting EGLN3 for proteasomal degradation. A RBE cells stably expressing pCDH and Flag-USP9X were subjected to analyze by immunoblotting using indicated antibody. B RBE cells stably expressing shNC, shUSP9X #1 and shUSP9X #2 were analyzed by immunoblotting. C RBE cells stably expressing pCDH, USP9X, shEGLN3 #1, shEGLN3 #2 alone or in combination were subjected to analyzed by immunoblotting using indicated antibody. D RBE cells stably expressing pCDH, USP9X, shEGLN3 #1, shEGLN3 #2 alone or in combination were subjected to analyze by flow-cytometry. E RBE cells stably expressing pCDH, USP9X, shEGLN3 #1, shEGLN3 #2 alone or in combination were subjected to analyze by Immunofluorescence laser confocal. Scale bars, 20 μm. ***, p < 0.001

Back to article page

Journal of Biomedical Science

ISSN: 1423-0127