Fig. 3

X-PCI-CT images of a healthy sample compared with corresponding histological images. A superficial layer and B middle/deep layer (TOMCAT beamline, voxel size 0.325 × 0.325 × 0.352 µm3); A.1 + B.1 + D + E nano-holotomography images from ID16A (0.1 × 0.1 × 0.1 µm3 voxel size); F Z-projection X-PCI-CT image of the tidemark (ID17, ESRF, 0.7 × 0.7 × 0.7 µm3 voxel size); C Histological images (scale bar 200 µm) of superficial, middle and deep layers (Masson Goldner trichrome staining: cell nuclei brown/black, cytoplasm red, collagen and proteoglycans green); G-I Histological images (scale bar 50 µm) of superficial, middle and deep layers (Masson Goldner trichrome staining)