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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Multiscale X-ray phase contrast imaging of human cartilage for investigating osteoarthritis formation

Fig. 6

X-PCI-CT images of healthy sample A-G (sagittal reformation) and degenerated sample HO; images acquired at the ID17 beamline with an effective voxel size of 0.7 × 0.7 × 0.7 µm3. In the center column of the panel: a histological H&E stained slice. F + G + M + O Layered X-PCI-CT images (i.e. Z-projections) from the upper and lower middle layers (minimum pixel values layered of 200 slices). A superficial layer; B part of the superficial and middle layers; C part of the middle and deep layers; D deep layer; E deep layer with adjusted gray level for tidemark visualization; F chondrocytes are partially aligned in an arc to support the superficial layer; G chondrocytes are oriented vertically in the transition zone between middle and deep layer along the fiber orientation. H superficial layer with uneven surface; I middle layer; J middle layer with a fissure; K deep layer; L deep layer with adjusted gray level for tidemark visualization; M superficial layer with rough surface area, chondrocytes are aligned in arc to support the existing superficial layer; G chondrocytes are oriented vertically in the transition zone between middle and deep layer along the fiber orientation

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